Research Updates, Santalum|

Abstract

Santalum album (Indian sandalwood) is an important tropical evergreen tree, known for its fragrant heartwood-derived essential oil and its valuable carving wood. Here, we applied an integrated genomic, transcriptomic, and proteomic approach to assemble and annotate the Indian sandalwood genome. Our genome sequencing resulted in the establishment of a draft map of the smallest genome for any woody tree species to date (221 Mb). The genome annotation predicted 38,119 protein-coding genes and 27.42% repetitive DNA elements. In-depth proteome analysis revealed the identities of 72,325 unique peptides, which confirmed 10,076 of the predicted genes. Addition of transcriptomic and proteogenomic approaches resulted in the identification of 53 novel proteins and 34 gene-correction events that were missed by genomic approaches. Proteogenomic analysis also helped in reassigning 1,348 potential non-coding RNAs as bona fide protein-coding mRNAs. Gene expression patterns at RNA and protein levels indicated that peptide sequencing was useful in capturing proteins encoded by nuclear and organellar genomes alike. Mass spectrometry-based proteomic evidence provided an unbiased approach towards identification of proteins encoded by organellar genomes. Such proteins are often missed in the transcriptome dataset due to enrichment of only mRNAs that contain poly-A tails. Overall, use of integrated -omic approaches enhanced the quality of assembly and annotation of this non-model plant genome. Availability of genomic, transcriptomic, and proteomic data will enhance genomics-a.

Reference: Mahesh, H. B., P. Subba, J. Advani, M. D. Shirke, R. M. Loganathan, S. Chandana, S. Shilpa, O. Chatterjee, S. M. Pinto and K. Prasad (2018). “Multi-omics driven assembly and annotation of the sandalwood (Santalum album) genome.” Plant physiology: pp. 01764.02017.

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